PCR Quantitative real-time PCR

Get tips on using Mastermix 16S Complete to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using QuantiTect Virus Kit (1000) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QuantiTect Virus +ROX Vial Kit (1000) to perform PCR Quantitative real-time PCR - Viral

Get tips on using FastLane Cell SYBR Green Kit (200) to perform PCR Quantitative real-time PCR - Viral

Get tips on using Fast SYBR™ Green Master Mix to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using LightCycler® 480 SYBR Green I Master to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Brilliant II SYBR Green qPCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using LightCycler® FastStart DNA Master SYBR Green I to perform PCR Quantitative real-time PCR - Mammalian DNA

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.